Electrodialysis cation exchange membranes were modified by incorporating [graphene oxide (GO)-co-Ag] composite nanoplates. The [GO-co-Ag] composite nanoplates were prepared by magnetron sputtering technique assisted with plasma treatment. XRD pattern and SEM image confirmed [GO-co-Ag] formation decisively. SEM, EDX, and SOM images showed relatively uniform surface for prepared membranes. Use of [GO-co-Ag] caused to formation of a compact structure for the blended membranes. The cross-sectional SEM images exhibited a specific direction for [GO-co-Ag], which at nanoscale position. The membrane surface wettability was improved by incorporating of [GO-co-Ag]. Membrane water content, transport number, and permselectivity were enhanced initially by utilizing of [GO-co-Ag] up to 0.5 wt% and then decreased by more concentration. Flux and E-conductivity enhanced sharply by using of [GO-co-Ag]. Use of [GO-co-Ag] into membrane matrix also caused to enhancement of mechanical resistance whereas declines the chemical oxidative stability. Results showed good antibacterial ability for blended membranes in E. coli removal.