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Seyed Mohammadali Shariatzadeh

Seyed Mohammadali Shariatzadeh

Academic rank: Professor
ORCID: https://orcid.org/0000-0002-2395-8057
Education: PhD.
ScopusId: 15133044400
Faculty: Science
Address: Arak University
Phone:

Research

Title
Angiogenic and Restorative Abilities of Human Mesenchymal Stem Cells Were Reduced Following Treatment With Serum From Diabetes Mellitus Type 2 Patients
Type
JournalPaper
Keywords
HUMAN MESENCHYMAL STEM CELLS; DIABETES MELLITUS TYPE 2; DIFFERENTIATION AND PARACRINE ANGIOGENESIS PROPERTIES; RESTORATIVE ABILITY
Year
2017
Journal Journal of Cellular Biochemistry
DOI
Researchers jafar rezaie ، Malek Soleimani mehranjani ، reza rahbarghazi ، Seyed Mohammadali Shariatzadeh

Abstract

This experiment investigated the impact of serum from patients with type 2 diabetes mellitus on the angiogenic behavior of human mesenchymal stem cells in vitro. Changes in the level of Ang-1, Ang-2, cell migration, and trans-differentiation into pericytes and endothelial lineage were monitored after 7 days. The interaction of mesenchymal stem cells with endothelial cells were evaluated using surface plasmon resonance technique. Paracrine restorative effect of diabetic stem cells was tested on pancreatic b cells. Compared to data from FBS and normal serum, diabetic serum reduced the stem cell survival and chemotaxis toward VEGF and SDF-1a (P <0.05). Diabetic condition were found to decline cell migration rate and the activity of MMP-2 and -9 (P <0.05). The down-regulation of VEGFR-2 and CXCR-4 was observed with an increase in the level of miR-1-3p and miR-15b-5p at the same time. The paracrine angiogenic potential of diabetic stem cells was disturbed via the changes in the dynamic of Ang-1, Ang-2, and VEGF. Surface plasmon resonance analysis showed that diabetes could induce an aberrant increase in the interaction of stem cells with endothelial cells. After treatment with diabetic serum, the expression of VE-cadherin and NG2 and ability for uptake of Dil-Ac-LDL were reduced (P <0.01). Conditioned media prepared from diabetic stem cells were unable to decrease fatty acid accumulation in b-cells (P <0.05). The level of insulin secreted by b-cells was not affected after exposure to supernatant from diabetic or non-diabetic mesenchymal stem cells. Data suggest diabetes could decrease angiogenic and restorative effect of stem cells in vitro