Nowadays the importance of biological cells in life activities is not hidden to anyone. There is an increasing need to understand the biological processes and since the experiments which consider a group of cells to be analyzed can only give average biological results, research on single cell could be really beneficial. So single cell analysis has become an interesting topic in recent years. In such an analysis, one of the most important steps could be the ability to transport the single cell to exact locations. In this paper, the process of manipulation of spherical yeast cells based on atomic force microscopy (AFM) has been investigated. The dynamic governing equations have been considered and a simulation based on them gave the results of the analysis. In order to verify the obtaining results, a comparison has been made with an experimental work of another group. The simulation results show that the critical sliding force in manipulation process of yeast cell is 5.648 micro N and in the experiment this force was 6.661 micro N. So it seems that there is a negligible difference between these two amounts and the results of the simulation are correct. The biological liquid is ethanol in all the parts, but a comparison has been made between three liquids (ethanol, methanol and blood) in order to investigate the effect of environment on the results. The results show that the viscosity of the environmental liquid has more effect rather than its surface tension. The comparison between three important contact theories (JKR, DMT and Hertz) shows that there is a small difference between the critical times and forces of them. Also the contact depth and contact radius of the three models are compared to each other and they also confirmed the small differences between these three models.
