Different traditional medicines have less solubility and low availability in the body. To enhance these properties new technologies are being introduced. Silymarin is a well-known antioxidative drug used against different diseases but has less solubility and bioavailability. In this work to enhance these properties it is loaded in a Cordia myxa plant extract encapsulated green fabricated ZIF-8 framework (CME@ZIF-8). The confirmation of the Sily@ZIF-8 nano drug was done by UV, XRD, FTIR, SEM, TEM, EDS, and ζ-potential. Further, the thermal stability of the samples was analyzed using thermogravimetric analysis. The average measured crystal size from XRD and average grain size of Sily-ZIF-8 nano drug from SEM histogram analysis was 17.23 nm and 515 nm respectively. The in vivo antioxidative potential in the brain and spleen of the prepared nano drug in comparison with pure silymarin was checked in CCl4 intoxicated albino rats with different dosing patterns of 500, 1000, and 1500 μg/kg BW. CCl4 increased TBARS and ROS levels, which induced DNA damage and reduced the CAT, POD, and SOD levels in these tissues. Treating rats with Sily@ZIF-8 restored these biochemical parameters to the normal level. Moreover, the histopathological studies of these tissues demonstrated that Sily@ZIF-8 protected the rat’s brain and spleen tissues from oxidative damage and restored normal functions of these tissues. Furthermore, the COMET assay depicted that the level of DNA damage was also mitigated with Sily@ZIF-8 nano-drug. This study suggests that the Sily@ZIF-8 nano drug protects different tissues possibly by mitigating oxidative stress more efficiently than the traditional drug silymarin and could be used in the future.
