The aim of current study was to prepared zinc oxide nanofertilzers by ecofriendly friendly, economically feasible, free of chemical contamination and safe for biological use.The study focused on crude extract of Withania coagulans as reducing agent for the green synthesis of ZnO nanoparticles. Biosynthesized ZnO NPs were characterized by UV–Vis spectroscopy, XRD, FTIR and GC–MS analysis. However, zinc oxide as green Nano fertilizer was used to analyze responses induced by diferent doses of ZnO NPs [0, 25, 50,100, 200 mg/l and Zn acetate (100 mg/l)] in Triticum aestivum (wheat). The stimulatory and inhibitory efects of foliar application of ZnO NPs were studied on wheat (Triticum aestivum) with aspect of biomass accumulation, morphological attributes, biochemical parameters and anatomical modifcations. Wheat plant showed signifcant (p < 0.01) enhancement of growth parameters upon exposure to ZnO NPs at specifc concentrations. In addition, wheat plant showed signifcant increase in biochemical attributes, chlorophyll content, carotenoids, carbohydrate and protein contents. Antioxidant enzyme (POD, SOD, CAT) and total favonoid content also confrmed nurturing impact on wheat plant. Increased stem, leaf and root anatomical parameters, all showed ZnO NPs mitigating capacity when applied to wheat. According to the current research, ZnO NPs application on wheat might be used to increase growth, yield, and Zn biofortifcation in wheat plants.
