2024 : 12 : 27
Mansour Ghorbanpour

Mansour Ghorbanpour

Academic rank: Professor
ORCID: https://orcid.org/0000-0002-4790-2701
Education: PhD.
ScopusId: 55220558500
HIndex:
Faculty: Agriculture and Environment
Address: Arak University
Phone:

Research

Title
Exposure to single-walled carbon nanotubes diferentially afect in vitro germination, biochemical and antioxidant properties of Thymus daenensis celak. seedlings
Type
JournalPaper
Keywords
SWCNTs, TEM, Thyme, Germination, DPPH, Toxicity
Year
2023
Journal BMC Plant Biology
DOI
Researchers Saba samadi ، Mohammad Jamal Saharkhiz ، Majid Azizi ، Leila Samiei ، Mansour Ghorbanpour

Abstract

Carbon nanomaterials such as single-walled carbon nanotubes (SWCNTs) ofer a new possibility for phyto-nanotechnology and biotechnology to improve the quality and quantity of secondary metabolites in vitro. The current study aimed to determine the SWCNTs efects on Thyme (Thymus daenensis celak.) seed germination. The seedlings were further assessed in terms of morphological and phytochemical properties. Sterile seeds were cultured in vitro and treated with various concentrations of SWCNTs. Biochemical analyses were designed on seedling sample extracts for measuring antioxidant activities (AA), total favonoids (TFC) and phenolic contents, and the main enzymes involved in oxidative reactions under experimental treatments. The results indicated that an increase in SWCNTs concentration can enhance the total percentage of seed germination. The improvement was observed in samples that received SWCNTs levels of up to 125 µg ml−1, even though seedling height and biomass accumulation decreased. Seedling growth parameters in the control samples were higher than those of grown in SWCNT-fortifed media. This may have happened because of more oxidative damage as well as a rise in POD and PPO activities in tissues. Additionally, secondary metabolites and relevant enzyme activities showed that maximum amounts of TPC, TFC, AA and the highest PAL enzyme activity were detected in samples exposed to 62.5 µg ml−1 SWCNTs. Our fndings reveal that SWCNTs in a concentration-dependent manner has diferent efects on T. daenensis morphological and phytochemical properties. Microscopic images analysis revealed that SWCNTs pierce cell walls, enter the plant cells and agglomerate in the cellular cytoplasm and cell walls. The fndings provide insights into the regulatory mechanisms of SWCNTs on T. daenensis growth, germination and secondary metabolites production.