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Malek Soleimani mehranjani

Malek Soleimani mehranjani

Academic rank: Professor
ORCID: https://orcid.org/0000-0003-4822-2617
Education: PhD.
ScopusId: 6505623754
HIndex:
Faculty: Science
Address: Arak University
Phone:

Research

Title
The effect of kisspeptin on the maturation of human ovarian follicles in culture following vitrification-thawing processes
Type
JournalPaper
Keywords
BMP15, follicle maturation, GDF 9, human ovarian cryopreservation, kisspeptin
Year
2023
Journal JBRA Assisted Reproduction
DOI
Researchers Anahita Tavakoli ، Malek Soleimani mehranjani ، Fereshteh Aliakbari

Abstract

Objective: Ovarian cryopreservation is one of the effective methods to preserve fertility for cancer patients. Still, this approach has some problems, namely ROS, resulting in adverse effects on oocytes and ovarian follicles. Kisspeptin as an antioxidant to control ovarian function, directly or indirectly. In this study, the effect of kisspeptin on follicle maturation was evaluated in culture following ovarian cryopreservation. Methods: Ovarian tissue samples of women between 20 and 35 years old (n=12) were laparoscopically collected. The samples were randomly divided into four groups: 1) control, 2) vitrification, 3) vitrified+1μM kisspeptin, and 4) vitrified+10μM kisspeptin. After vitrification and thawing processes, the tissues were cultured in DMEM medium for 7 days. H&E staining for histological evaluation, Real- Time PCR for GDF9 and BMP15 gene expression, and immunohistochemical staining for GDF9 and BMP15 protein expression were performed. Results: In the vitrification group, ovarian tissue morphology was incoherent, and more primordial follicles than other follicle types were found. The expression of GDF9 and BMP15 genes and proteins were significantly decreased in this group compared with other groups (p<0.05). In the vitrification groups with kisspeptin (1 and 10 μM), the number of primary and secondary follicles was more than in the vitrification group. Besides, the expression of these genes and proteins was dramatically elevated in the vitrification groups with kisspeptin compared to the vitrification group alone (p<0.05). Conclusions: It seems that kisspeptin is an effective substance to improve the quality of the human ovarian cryopreservation medium by improving follicle maturation.