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Javad Sargolzaei

Javad Sargolzaei

Academic rank: Assistant Professor
ORCID: https://orcid.org/0000-0001-5366-2014
Education: PhD.
ScopusId: 57191348268
HIndex:
Faculty: Science
Address: Arak University
Phone:

Research

Title
Gallic Acid Ameliorates Cadmium Effect on Osteogenesis by Activation of Alkaline Phosphatase and Collagen Synthesis
Type
JournalPaper
Keywords
Cadmium, Gallic Acid, Mesenchymal Stem Cell, Osteoblasts
Year
2023
Journal Cell Journal (yakhteh)
DOI
Researchers Mohammadhossein Abnosi ، Javad Sargolzaei ، Farshid Nazari

Abstract

Objective: We previously reported that cadmium (Cd) inhibits osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). In addition, gallic acid (GA) improves BMSC differentiation. Here, we aim to study the ability of GA to prevent osteogenic inhibition induced by Cd. Materials and Methods: In this experimental study, BMSCs were extracted and purified from Wistar rats and their viability was determined in the presence of Cd and GA. The results indicated that 1.5 μM Cd and 0.25 μM of GA were appropriate for further investigation. After 20 days in osteogenic medium, matrix production was analysed by alizarin red, calcium content, and alkaline phosphatase (ALP) activity. Osteogenic-related genes and collagen 1A1 (COL1A1) protein expressions were investigated. The preventive effect of GA on oxidative stress and metabolic change induced by Cd was estimated. Results: GA counteracted the inhibitory effect of Cd on matrix production and significantly (P=0.0001) improved the osteogenic differentiation ability of BMSCs. Also, GA prevented the toxic effect of Cd on osteogenic-related gene expressions and nullified the reducing effect of Cd on COL1Al and ALP activity. A significant reduction (P=0.0001) in malondialdehyde and lactic acid concentration showed that GA counteracted both oxidative stress and metabolic changes caused by Cd. Conclusion: GA prevented the toxic effect of Cd, an environmental pollutant and a factor in osteoporosis.