In recent years, efective plant compounds including menthol have been investigated as anti-cancer agents. However, since these organic compounds have low cell penetration, it is possible to greatly increase the possibility of cell permeability by combining them with metallic nanoparticles (NPs). The aim of this study was the synthesis and characterization of menthol conjugated magnetic Iron oxide NPs and evaluation of their cytotoxic activity on GC cell lines. Iron oxide (Fe3O4) NPs were synthesized by co-precipitation method, coated with glucose, and fnally conjugated with menthol. Fourier-transform infrared spectroscopy (FTIR), Dynamic Light Scattering (DLS), X-ray difraction (XRD), energy dispersive X-ray spectroscopy (EDS), Zeta-potential, scanning electron microscope (SEM) and transmission electron microscope (TEM) techniques were used to investigate the chemical and physical properties of synthesized Fe3O4@Glu-menthol magnetic nanoparticles (MNPs). The anticancer efects of MNPs on AGS cell line were assessed using MTT assay, fow cytometry, and caspase-8 gene expression analysis. The size of the synthesized NPs ranges from 20 to 60 nm with elemental compositions of the carbon, oxygen, and iron. The MTT assay showed that the IC50 value of Fe3O4@Glu-menthol MNPs was equal to 252 μg/mL for Gastric cancer cell line. The results of the fow cytometry analysis showed that about 12.1% of cells had early apoptosis, 20.6% had late apoptosis and 11.2% were in necrosis condition. In addition, in cells exposed to Fe3O4@Glu-menthol MNPs, the expression of CASP8 was 1.6 times more compared than untreated cells. In conclusion and based on the results, Fe3O4@ Glu-menthol MNPs can induce programmed cell death in Gastric cancer cell line.