Background and Aim: Rheumatoid arthritis (RA) is a chronic autoimmune disorder primarily affecting the joints. Interleukin-10 (IL-10) is an important immunoregulatory cytokine involved in regulating the immune response and inflammation. Polymorphism in the promoter region of the IL-10 can affect the expression of the gene. This study aims to investigate the association of human IL-10 rs1800896 (-1082 A/G), rs1800871 (-824 C/T), and rs1800872 (-597 C/A) promoter single nucleotide polymorphisms (SNPs) with susceptibility to RA in the Iraqi women population. Methods: Here, 280 RA female patients who referred to the AL-Hussein Teaching Hospital in Karbala, Iraq between November 2022 to April 2023, and 120 healthy agematched subjects were examined. The two groups were genotyped for rs1800896, rs1800871, and rs1800872 by tetra-primer ARMS–PCR. Statistical analyses were performed using IBM SPSS windows version 25 Results: All the genotypes did not deviate from Hardy-Weinberg Equilibrium (HWE) expectations in both groups. No positive association was found between allele and genotype frequencies of three SNPs and the incidence of rheumatoid arthritis (all p>0.05). Only the ACC haplotype showed an association with disease susceptibility (pvalue=0.0004). Conclusion: Based on the results, promoter SNPs of the IL-10 gene cannot be used as a suitable marker to determine the susceptibility to rheumatoid arthritis in the Iraqi women population and to prevent its progression
