2024 : 10 : 5
Alireza Karimi

Alireza Karimi

Academic rank: Professor
ORCID: https://orcid.org/0000-0001-5006-8642
Education: PhD.
ScopusId: 57217189368
HIndex:
Faculty: Science
Address: Arak University
Phone:

Research

Title
Emergence of in vitro antimicrobial photodynamic activities of perylene-dopamine and enhancement of its phototoxicity properties via chelating with FeIII and encapsulating in CMC-based safe hydrogel film
Type
JournalPaper
Keywords
Antimicrobial and anticancer photodynamic therapy Perylene dopamine/FeIII complexes Antioxidant activity S. aureus bacterium Biocompatible CMC-based film
Year
2024
Journal Journal of Photochemistry & Photobiology, A: Chemistry
DOI
Researchers Bahareh Rostaminejad ، Alireza Karimi ، Mohammad Dinari ، Mahnaz Hadizadeh

Abstract

In the present work, we achieved a multifunctional antioxidant, antimicrobial, and anti-cancer platform by designing and encapsulating phenolic PDT drug/FeIII complex (PBI-DOPA/FeIII) into the CMC-based biocompatible hydrogel film matrix. The photosensitive CMC-CA/PBI-DOPA/FeIII system was synthesized via a one-pot process, in which the PBI-DOPA/FeIII complex can be trapped during the CMC cross-linking process by citric acid. The phenolic groups of PBI-DOPA can play a dual role, either being chelated by iron ions or otherwise imparting antioxidant properties to the hydrogel film. The presence of metal ions exerts an advantageous effect on oxygen production and phototoxicity of the photosensitive system by facilitating ISC (intersystem crossing). In vitro PDT potential of CMC-CA/PBI-DOPA/Fe (III) film and free PBI-DOPA was assessed on cancerous MCF-7 and non-cancerous MCF10A cell lines. As we expected, the PDT efficiency of encapsulated PBI-DOPA was dramatically enhanced compared to the free form and could induce up to 65% cancer cell apoptosis. Moreover, the photosensitive system proved effective antimicrobial activity against S.aureus (ATCC 6538) that greatly killed and inhibited the growth of microbes. Therefore, the innovative multifunctional CMC-CA/PBI-DOPA/Fe (III) hydrogel film with superior antioxidant, antimicrobial, and anticancer properties, which achieved enhanced cytotoxicity through PBI-DOPA/FeIII complexes, could serve as a promising efficient weapon in PDT against breast cancer and bacterial infections by minimizing side effects.