In this study, chitosan/polylactide scaffolds reinforced with nano-calcium phosphate (average crystallite size of 16.5 nm) (CP) were fabricated to create a material with excellent properties for bone tissue engineering applications via freeze-casting method. The structural and mechanical properties of nanocomposite scaffolds were studied by increasing amount of chitosan/poly lactide ratio and nano-CP content in both dry and hydrate states, which reflected the exact status of scaffolds in a real biomechanical environment. The morphologies of the nanocomposite scaffolds were viewed using scanning electron microscopy (SEM) and all the scaffolds exhibited a high porosity (up to 92 %) with open pores of 38–387 μm average diameters, which decreased with increased chitosan/polylactide ratio and nano-CP content. Also, SEM photograph of the cross-sectional area of the scaffold showed nano-CP was dispersed all over the polymer matrix thoroughly. The results of mechanical tests showed that the compressive modulus (E) and compressive stress (σ) enhanced, when chitosan and nano-CP increased. X-ray diffraction analysis indicated typical chitosan, polylactic acid and nano-CP peaks and showed that the increase in nano-CP weight percentage increased its peak intensities. In addition, the effect of pore-size distributions of the scaffolds with the same composition was studied in relation to mechanical properties. The results showed substantial differences in the pore-size distributions of scaffolds with the same composition prepared, which have no effect on their dry states.
